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REVIEW

Biotechnological Strategies for Cultured Poultry Meat Biofabrication Through Induced Pluripotent Stem Cell Reprogramming and CRISPR-Cas9-Mediated Genome Editing.

The review examines how induced pluripotent stem cells and CRISPR-Cas9 could be combined to produce cultivated chicken. It outlines reprogramming Gallus gallus somatic cells with Oct4, Sox2, Klf4 and c-Myc, editing genes to improve muscle formation, growth, nutrition and disease resistance, then expanding cells without animal-derived components and differentiating them into muscle. Scaffolds and perfused bioreactors would form three-dimensional tissue while regulating oxygen and nutrients. The authors report potential for antibiotic-free poultry with lower environmental impacts and scalable production.

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Kinetic phenotyping of Fusarium venenatum A3/5 across waste-relevant carbon substrates

Fusarium venenatum A3/5 used waste-relevant carbon sources in markedly different ways, affecting feedstock choices for mycoprotein production. Researchers combined microlitre cultures, optical-density calibration, UHPLC time series and Gompertz models. Glucose and sucrose enabled fast growth but produced low biomass yields and substantial ethanol. Fructose gave the highest yield, at 0.49 g/g, while lactose consumption reached only 34% after 234.5 hours. An expired functional drink produced 18.37 g/L biomass, versus 12.49 g/L for a matched synthetic control. Beverage waste was the strongest tested feedstock, but evidence covers one real waste stream.

Science digest

Toward Rational Design of Precision-Fermented Milk Proteins: Integrating Cross-Species Selection, Post-Translational Modification, and AI Optimization.

A review maps how milk proteins from humans, cows, goats and camels could be chosen and made through precision fermentation, addressing design gaps in target selection and protein processing. It compares sequences, structures and post-production modifications, then assesses host engineering, scale-up, purification and AI methods. Human proteins suit infant nutrition and immune uses, while ruminant proteins fit current expression systems better. α-Lactalbumin and β-lactoglobulin need fewer modifications than caseins and lactoferrin. AI cannot yet optimise yield, folding and modification accuracy together, leaving scale-up, glycoengineering and regulation unresolved.

Electrohydrodynamics and Fluid Dynamics

Electrosprayed edible microcarriers based on gelatin-coated alginate for muscle cell culture

Gelatin-coated alginate microcarriers supported muscle cell culture in static and agitated systems, suggesting an edible scaffold for cultivated meat production. Researchers electrosprayed spherical, 242 µm alginate particles, coated them with gelatin, and seeded them with mouse myoblasts and immortalised bovine satellite cells at two densities for seven days. Both cell types remained viable and proliferated under static conditions, with metabolic activity similar to 2D cultures. Agitation slightly reduced proliferation, but metabolic activity and carrier structure remained stable. The early shake-flask evidence warrants further scale-up testing.