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RESEARCH ARTICLE

Food-grade utilization of bovine slaughterhouse by-products for developing cost-effective media supplements in cultivated meat applications

Bovine slaughter by-products were assessed as food-grade media ingredients for cultivated meat. The review examined muscle growth and repair biology, satellite cells, myogenic regulators, drawbacks of fetal bovine serum, and alternatives including recombinant proteins, food-derived components, and adult livestock serum. Compositional analysis found that blood and organs provide proteins, amino acids, fats, and minerals relevant to muscle cell growth and maturation. Plasma and serum had different functional profiles, while liver, heart, spleen, tongue, and other tissues contained varying nutrients that could support lower-serum or serum-free media.

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