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RESEARCH ARTICLE

Rapid Extrusion of Myotube-Scale Alginate Fibers for Structured Cultivated Meat.

Researchers tested PASTA, a pressurized liquid–liquid extrusion process, to make edible alginate fiber scaffolds for structured cultivated meat. They used below-boiling hydrothermal treatment to tune alginate viscosity, surface charge and gel mechanics, then extruded fibers and cultured murine C2C12 muscle precursor cells on peptide-functionalized scaffolds. Fibers were 10–42% narrower than the outlet. Cells attached, multiplied, aligned and matured along the fibers, with DNA rising 3.5-fold in four days, viability at day five, and molecular and staining evidence of muscle differentiation.

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