Valorisation of Fermentation Side-Stream for Waste-to-Mycoprotein: Nutrient Composition, Metabolic Insights and Process Optimisation
27 September 2026 · Biofuel production and bioconversion · Microbial Metabolic Engineering and Bioproduction · Fungal Biology and Applications
Researchers assessed whether centrate left from industrial Fusarium venenatum fermentation could supply nutrients for producing additional fungal biomass. After analysing its composition, they created a synthetic equivalent and tested nutrient combinations in 46 runs using Box-Behnken experimental design and response-surface modelling. Biomass yields varied from 0.22 to 3.87 g/L, with glucose and ammonia exerting the largest, nonlinear effects. Optimisation predicted 4.17 g/L and produced 3.99 g/L experimentally. Carbon conversion reached 29.02%, supporting centrate’s use for nutrient recovery and mycoprotein production.