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RESEARCH ARTICLE

A Streamlined Workflow for the Isolation of Bovine Satellite Cells From Muscle Tissue.

Bovine satellite cells were isolated through a simpler process intended to cut the labour and cost of preparing cells for cultivated meat and muscle research. Researchers electrically minced muscle, then used magnetic sorting against integrin alpha 7. Pax7 staining and differentiation tests assessed the cells. The authors report 49.39 ± 14.70 thousand cells per gram, with 98 ± 1.34% Pax7 positivity and a 94.39 ± 3.58% differentiation index. The method produces enriched, functional early-passage cultures with less specialised equipment.

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