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RESEARCH ARTICLE

Alterations of Fusarium venenatum mycelium upon sequential processing mediate the interfacial behavior and emulsion stability.

Sequential nucleic acid removal and sterilisation weakened the emulsifying function of Fusarium venenatum mycelium, exposing a trade-off between food safety and product performance. Researchers measured composition, surface structure, mechanics, interfacial activity and emulsion stability after each processing step. Protein content fell from 50.96% to 43.47%, while atomic force microscopy showed rougher, flatter and less uniform surfaces. Untreated mycelium stabilised emulsions during storage, heating and salt exposure. Nucleic acid-removed material remained functional at higher concentrations, but sterilised material coalesced most readily. Processing conditions therefore need tighter control.

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