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RESEARCH ARTICLE

Expanding the Genetic Toolkit of Pichia kudriavzevii E1 for Enhanced Mycoprotein Production

Researchers built genetic tools for Pichia kudriavzevii E1 that enabled higher protein production and more controlled strain engineering. They identified a genomic sequence that supports stable plasmid replication, then used transcriptome data to assemble a library of native promoters. PkTDH3 produced the strongest activity and maintained expression better than the Saccharomyces cerevisiae ScTDH3 promoter. Random changes to its upstream activation region widened the available expression range. Mutant strains made with the library had increased protein content, showing its practical value for mycoprotein development.

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