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Induced Pluripotent Stem Cells in Non-Model Species: Applications and Challenges

A review examines induced pluripotent stem cells in non-model species, where limited genomic data, reagents and culture methods constrain research relevant to conservation, medicine, agriculture and toxicology. It assesses reprogramming methods and applications including genetic rescue, laboratory-derived gametes, species-specific disease models, climate tolerance, disease resistance, cultivated meat and pollution testing. The authors find that vertebrates share the main biological machinery for pluripotency, while practical tools remain scarce. Progress depends less on biological feasibility than on building species-specific research infrastructure.

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A Sustainable Approach for the Use of Plant Proteins as Functional Ingredients in Dairy Alternatives.

A review assesses plant proteins for dairy alternatives, linking nutritional quality, processing and regulation to their use in specific products. It compares legumes, cereals, pseudocereals, oilseeds and nuts against milk proteins using DIAAS, digestibility and antinutrient content. It also examines extraction methods, naming rules and shifts towards precision fermentation and mycoprotein. The authors find that the balance of 7S and 11S globulins predicts emulsifying, foaming and gelling better than source or purity. Yet inconsistent assays and extraction damage cloud comparisons, so formulation choices need application-specific evidence.

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Enzyme kinetics and engineering strategies for sustainable alternative protein production: From plant and microbial systems to cultivated meat.

The chapter examines how enzyme kinetics and engineering could improve proteins made from plants, microbes, algae, precision fermentation and animal cell culture, a key issue for product quality and scale-up. It reviews inhibition, catalysis and models for mixed protein substrates, alongside uses of transglutaminases, carbohydrases and lipases. It also assesses rational design, immobilisation, directed evolution, computational methods and links between kinetic models and bioreactors. The authors conclude that combining these tools can improve food properties and production efficiency, though the abstract reports no new experimental evidence.

Science digest

Cultivated meat at industrial scale: Hygiene, externalized burdens, and the social conditions of meat wholesomeness.

Industrial-scale cultivated meat is assessed as a more controlled alternative to intensive livestock, with consequences for food safety, ethics and fairness. The commentary compares livestock’s environmental footprint, animal harm, microbial hazards, dangerous work and uneven community exposure with cell culture using enclosed systems and batch tracking. It identifies possible gains in hygiene, lower reliance on slaughter and inclusion of people who reject conventional meat. Yet energy use, growth media, scaling, concentrated ownership, cost and public confidence remain unresolved, so tighter control alone cannot ensure wholesome or just meat.