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Methylotrophic yeast engineering for secreted protein production via precision fermentation: food ingredient proteins and synthetic biology strategies.

A review examines how methanol-using yeasts can make and release food proteins, a key constraint on the cost, output and function of precision-fermented ingredients. It centres on Komagataella phaffii and covers dairy proteins, inputs for plant-based and cultivated meat, and sweet proteins. The authors assess genome editing, gene-control systems, secretion-pathway changes and glycosylation engineering as routes to improve yield and product quality. They conclude that progress also depends on linking strain design with industrial process development.

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