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RESEARCH ARTICLE

Precision fermentation of κ-casein using Pichia pastoris .

Bovine κ-casein was secreted from the yeast Pichia pastoris, but degradation, incomplete release and extensive glycosylation limited yields, which could raise processing costs. Researchers compared strains carrying multiple gene copies, deleted yapsin 1 alone or with protease A, tested bioreactor cultures, examined periplasmic protein and tried cytoplasmic expression. More copies increased titres, yet the knockouts did not stop degradation. Cytoplasmic production neither raised output nor clearly preserved protein integrity. The host needs further engineering before κ-casein production can become economically viable.

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