Understanding Osmolalityas a Critical Process Parameterin Mammalian Muscle Cell Culture and Biomanufacturing
18 July 2026 · Viral Infectious Diseases and Gene Expression in Insects · thermodynamics and calorimetric analyses · Meat and Animal Product Quality
The review examines osmolality as a process variable in mammalian muscle culture and biomanufacturing. It combines physicochemical principles with evidence from muscle-lineage and wider mammalian bioprocess research, including limitations of calculation and measurement methods. Muscle cells operate within a narrow osmotic range, and deviations can alter growth, differentiation, and fusion. Medium ingredients share a limited osmotic allowance, while carbon dioxide and pH coupling, temperature, evaporation, scaffolds, and bioinks can cause drift. Tracking osmolality over time could improve control during scale-up.